Zentrale Abteilung für Elektronenmikroskopie
Imaging Core Facility

Research: Synaptic architecture at the nano-scale

Neuronal synapses are highly efficient and complex cellular signaling machineries that achieve remarkable precision in signal transmission for a prolonged period of time, in some cases throughout the lifetime of an animal. The importance of synaptic efficiency is mirrored by many neural diseases but in particular by synaptopathies where synaptic organization and function is disrupted. In order to provide reliable signaling synaptic vesicles have to be retained close to the presynaptic active zone, the domain where vesicles are docked and fuse with the membrane after Ca influx in a nano-domain through voltage gated channels.
How are synaptic vesicles kept coherently close to the active zone to maintain efficient signaling? To shed light onto this question our team focuses on the cellular architecture using a combination of genetic tools and imaging techniques. In particular we apply electron tomography as ultra high 3D resolution method to dissect components and function of synaptic architecture. We use a synergistic combination of two highly tractable models where they are most appropriate: The C. elegans neuromuscular junctions for efficient candidate identification and manipulation and the neuromuscular junctions of the zebrafish larva as vertebrate model to test for evolutionary conservation of function.

Team

Sebastian Britz (Master Student MSc Biology)

Frederik Helmprobst (PhD Student)

Sebastian Markert (PhD Student)

Christian Stigloher (Jun. Prof.)

Marlene Strobel (Master Student MSc Biology)

 

List of Publications

Address
University of Wuerzburg
Biocenter
Electron Microscopy
Hubland Campus South
Am Hubland
97070 Wuerzburg
Tel. +49 (0)931 31-89850
Fax: +49 (0)931 31-84252
E-Mail
Hubland Süd Hubland Nord Campus Dallenberg Fabrikschleichach Humangenetik Campus Medizin